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PTEN-P8

規格:

價格:

編號:B210387

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

PTEN-P8

商品貨號

B210387

Organism

Mus musculus, mouse

Tissue

Prostate epithelium

培養基

Product Format

frozen

Morphology

Epithelial-like

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Prostate cancer tumor

Age

10 month

Gender

Male

Applications

PTEN signaling pathways and prostate cancer

Shipping Information

frozen

Storage Conditions

liquid nitrogen vapor phase

Karyotype

Chromosome Count: near 6N (113-125)
Numerical Abnormalities: -1, -4, +10, +14, +15, +16, +17, -Y
Structural Abnormalities: Der(2)T(2;11), Del(7C), Del(8A3), Del(10A1), Del(13B), Del(14C), Del(15B)

Images

Derivation

Prostate cancer tissue dissected from 10-month, intact Pten.loxp/loxp;PB-Cre4+ mouse

Receptor Expression

androgen receptor +

Comments

PTEN mutations are one of the the most frequent genetic alterations found in human prostate cancers. This cell line, PTEN-P8, along with its isogenic partner, PTEN-CaP8 was generated to better understand the underlying molecular mechanisms of PTEN in prostate cancer progression and control, as well as the signaling pathways controlled by PTEN. PTEN-P8 is heterozygous for Pten deletion. Down-regulation of the Cre transgene produces very low to undetectable levels of Cre protein expression in this cell line. This cell line was generated from tissue that had not been subjected to hormone ablation therapy and are thus ideal for the study of human refractory prostate cancer formation, as many of the most well-studied human prostate cancer cell lines are from late-stage cancer tissue that has undergone such therapy.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco’s Modified Eagle’s Medium, (ATCC^® No. 30-2002). To make the complete growth medium, add the following components to 500 ml of the base medium:

fetal bovine serum (FBS; ATCC^® No. 30-2020) to a final concentration of 10%

25 µg/mL bovine pituitary extract (BPE)

5 µg/mL human recombinant insulin

6 ng/mL human recombinant epidermal growth factor (EGF)

Note: Do not filter complete medium

Subculturing

S/C when culture reaches ~70-85% confluence.

A subcultivation ratio of 1:20 to 1:50 is recommended.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces
of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted
microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8.0 X 103
to 1.0 x 104 viable cells/cm2 is recommended.
6. Incubate cultures at 37°C.

Renew medium every 2 to 3 days.

Culture Conditions

Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Population Doubling Time

approximately 14 hours

Name of Depositor

Dr. Jing Jiao & Dr. Hong Wu

Year of Origin

2004

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