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pHSY15
pHSY15
規格:
價格:
編號:B210686
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHSY15
商品貨號
B210686
Designations
pHSY15
GenBank Number

M97655

培養基
Species
Homo sapiens, human
Depositors
B Thony
Vector
Construct size (kb): 3.299999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PTS
Genomic copy number: unique
Gene name: 6-pyruvoyltetrahydropterin synthase
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q22.3-q23.3
Tissue: liver
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 0.635
Cross references: DNA Seq. Acc.: M97655
Insert lengths(kb): 0.6349999904632568
Tissue: liver
Gene product: 6-pyruvoyltetrahydropterin synthase [PTS]
Insert Size (kb)
0.635
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.7, 0.65; BglI--2.2, 1.15; HindIII--3.35; PstI--3.35; PvuII--2.35, 1.0.
Contains the complete coding sequence (nt 8-439).
Constructed by amplification using the following primers incorporating BamHI and EcoRI sites: PTPS11-5'CGGGATCCATATGAGCACGGAAGGTGGTG 3' and PTPS14-5'CGGAATTCAGGATGTATATTACAAGTT 3'.
References

Thony B, et al. Human 6-pyruvoyltetrahydropterin synthase: cDNA cloning and heterologous expression of the recombinant enzyme. Biochem. Biophys. Res. Commun. 189: 1437-1443, 1992. PubMed: 1282802

Beat Thony, personal communication

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