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Ca9-1
Ca9-1
規(guī)格:
價(jià)格:
編號(hào):B210737
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Ca9-1
商品貨號(hào)
B210737
Designations
Ca9-1
GenBank Number

U07561

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 45.16299819946289
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: svPHEP
Intact vector size: 9.201
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI ClaI
Polylinker sites: HindIII ClaI EcoRI BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L19900
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: unique
Gene name: gene X
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: lung carcinoma
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.962
Cross references: DNA Seq. Acc.: U07561
Nucleotides 1-35962 of the insert correspond to
nucleotides 1-35962 of U07561.
Insert lengths(kb): 35.96200180053711
Tissue: lung carcinoma
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb)
35.962
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI-->23.0; BglII--12.0, 6.6, 6.1, 5.8, 4.6 (doublet), 4.2, 2.8, 0.52; EcoRI--8.0, 7.0, 6.6, 4.9, 4.2 (doublet), 2.65, 2.15 (doublet), 1.55 (doublet);
HindIII--14.0 (doublet), 6.1, 2.55 (doublet), 2.3, 2.05, 1.15, 0.96, 0.74, 0.44; SstI--14.0, 10.5, 7.0, 5.8 (doublet), 3.8, 0.92.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The insert begins 8604 nt upstream of "gene X", includes all 8 exons of that gene (through nt 18583) and continues through exon 1b (29132-29267) of ABL1. A CML breakpoint occurs in the intergenic region.
References

Groffen J, et al. Isolation of human oncogene sequences (v-fes homolog) from a cosmid library. Science 216: 1136-1138, 1982. PubMed: 6281890

Morris CM, et al. Entire ABL gene is joined with 5'-BCR in some patients with philadelphia-positive leukemia. Blood 78: 1078-1084, 1991. PubMed: 1868241

Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185

Bruce A Roe, personal communication

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