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當(dāng)前位置: 首頁 > ATCC代理 > pPW71B [PW71B] B210756
pPW71B [PW71B]
pPW71B [PW71B]
規(guī)格:
價格:
編號:B210756
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pPW71B [PW71B]
商品貨號
B210756
Designations
pPW71B [PW71B]
Species
Homo sapiens, human
培養(yǎng)基
Applications
contains sequence useful for DNA diagnostics DNA Segment, single copy probes
Vector
Construct size (kb): 3.049999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D15S63
Gene name: DNA Segment, single copy probes
Contains complete coding sequence?: N
Chromosome: 15; Localization: 15 q11-q12
Type of DNA: genomic
Insert end: HaeIII
Insert end: HaeIII
Insert size (kb): 0.365
Cross references:
Insert lengths(kb): 0.3650000095367432
Gene product: DNA Segment, single copy probes [D15S63]
Target Gene: DNA Segment, single copy probes
Insert Size (kb)
0.365
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2.
Insert detects parent-of-origin specific DNA methylation patterns in the Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An imprinted HpaII and CfoI site in this region results in the maternal and paternal pattern.
Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of the maternal bands is indicative of Angelman syndrome. Because of the small probe size, low-stringent post-hybridization washes should be applied.
References

Dittrich B, et al. Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum. Mol. Genet. 2: 1995-1999, 1993. PubMed: 8111366

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