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pcD-hIL-4
pcD-hIL-4
規(guī)格:
價格:
編號:B210928
品牌:Mingzhoubio

標(biāo)準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pcD-hIL-4
商品貨號
B210928
Designations
pcD-hIL-4
GenBank Number

M13982

培養(yǎng)基
Species
Homo sapiens, human
Depositors
T Yokota
Vector
Construct size (kb): 3.839999914169312
Insert
DNA: cDNA
Insert lengths(kb): 0.7200000286102295
Tissue: helper T-cell 2F1 cell line, Con A-stimulated
Gene product: interleukin 4 [IL4]
Insert Size (kb)
0.720
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.8; HindIII--3.8; PstI--2.3, 1.6; ClaI--3.8; XhoI--2.8, 0.9.
Exact restriction sizes are as follows: BamHI--2.98 kb, 0.86 kb; HindIII--3.84 kb; PstI--2.27 kb, 1.57 kb; ClaI--3.84 kb; XhoI--2.92 kb, 0.92 kb.
The PstI site given in the paper at the 5' end does not exist. The plasmid contains the PstI site in bla and a PstI site in the insert.
Interleukin 4 and interleukin 5 are encoded in the same 345 kb NotI fragment.
Contains about 100 bp of poly(A).
The insert contains the complete coding sequence and includes NheI, EcoRV, PstI, PvuII, BglI, and EcoRI sites.
References

Chandrasekharappa SC, et al. A long-range restriciton map of the interleukin-4 and interleukin-5 linkage group on chromosome 5. Genomics 6: 94-99, 1990. PubMed: 2303264

Yokota T, et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 5894-5898, 1986. PubMed: 3016727

Takashi Yokota, personal communication

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