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B505
B505
規(guī)格:
價(jià)格:
編號(hào):B210932
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
B505
商品貨號(hào)
B210932
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with human luteinizing hormone (hLH) beta core fragment isolated from human pituitary extracts. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat. 5,976,876].
Genes Expressed
immunoglobulin; monoclonal antibody; against human luteinizing hormone (hLH) beta core fragment
Cellular Products
immunoglobulin; monoclonal antibody; against human luteinizing hormone (hLH) beta core fragment
Comments
Animals were immunized with human luteinizing hormone (hLH) beta core fragment isolated from human pituitary extracts. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The B505 antibody specifically binds to hLH beta core fragment without cross-reacting with hLH, hLH beta or human chorionic gonadotropin (hCG) beta core fragment [U.S. Pat. 5,976,876].
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG1 kappa
Name of Depositor
Trustees of Columbia University
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Canfield RE, et al. Antibodies specific for hLH beta core fragment. US Patent 5,976,876 dated Nov 2 1999

Birkin S, et al. Determination of the amount of hLH.beta. core fragment in a sample from a subject and uses thereof. US Patent 6,521,416 dated Feb 18 2003

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