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pHMANT [H20d]
pHMANT [H20d]
規格:
價格:
編號:B211000
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHMANT [H20d]
商品貨號
B211000
Designations
pHMANT [H20d]
GenBank Number

J02966

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.399999976158142
Tissue: skeletal muscle
Gene product: adenine nucleotide translocator 1 (skeletal muscle) [ANT1]
Insert Size (kb)
1.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Insert contains NcoI (2), SacII, PstI, BglII (2), HincII, and DraI sites. If the clone is digested with HindIII/PvuII, the insert is released with about 300 bp vector sequence.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; BglII--3.7, 0.56; ClaI--4.4; PstI--3.0, 1.15; HindIII--4.4; PvuII/HindIII--2.5, 1.7; BglI/HindIII--2.9, 1.0.
This contains the complete coding sequence. The initiation Met codon begins 104 nt from the 5' end in an NcoI site. There is an open reading frame of 891 nt followed by 299 nt 3' untranslated sequence and about 100 nt poly(A).
At the amino acid sequence level, this clone is more similar to a bovine adenine nucleotide translocator than to a human fibroblast adenine nucleotide translocator.
References

Neckelmann N, et al. cDNA sequence of a human skeletal muscle ADP/ATP translocator: lack of a leader peptide, divergence from a fibroblast translocator cDNA, and coevolution with mitochondrial DNA genes. Proc. Natl. Acad. Sci. USA 84: 7580-7584, 1987. PubMed: 2823266

Garrison JC, et al. Isolation of full-length cDNAs encoding abundant adult human skeletal muscle mRNAs. Gene 38: 177-188, 1985. PubMed: 3840762

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