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pBamRM5.0 [NEB699]
pBamRM5.0 [NEB699]
規格:
價格:
編號:B211305
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBamRM5.0 [NEB699]
商品貨號
B211305
Designations
pBamRM5.0 [NEB699]
GenBank Number

X55285

培養基
Species
Bacillus amyloliquefaciens (Fukumoto) Priest et al.
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
produces protein modification methylase BamHI
produces protein restriction endonuclease BamHI
Vector
Construct size (kb): 4.5
Insert
DNA: genomic
Insert lengths(kb): 1.799999952316284
Gene product: modification methylase BamHI [bamHIC]
Target Gene: modification methylase BamHI, restriction endonuclease BamHI
Insert Size (kb)
1.800
Media
ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of pBamRM5.0 give the following sizes (kb): HindIII/SmaI--5.0, 4.4; HindIII--5.0, 4.4; PvuII--4.5, 4.5, 0.4; EcoRI--9.5.
Restriction digests of pBamM1.8 give the following sizes (kb)(faint): HindIII/SmaI--4.4; HindIII--4.4; PvuII--2.4, 2.1; EcoRI--4.5.
References

Brooks JE. Method for producing the BamHI restriction endonuclease and methylase. US Patent 5,137,823 dated Aug 11 1992

Brooks JE, et al. Cloning the BamHI restriction modification system. Nucleic Acids Res. 17: 979-997, 1989. PubMed: 2537955

Brooks JE, et al. Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts. Nucleic Acids Res. 19: 841-850, 1991. PubMed: 1901989

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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