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U-CH1
U-CH1
規格:
價格:
編號:B211393
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
U-CH1
商品貨號
B211393
Organism
Homo sapiens, human
Tissue
sacral bone
培養基
Product Format
frozen
Morphology
mesenchymal like, with variable vacuoles
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
tumor, chordoma
Age
56 years
Gender
male
Ethnicity
Caucasian
Applications
Use as a model of chordoma which is a rare slow-growing tumor

The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.
Shipping Information
frozen
Storage Conditions
liquid nitrogen vapor phase
Images
Cell micrograph of ATCC CRL-3217 ( U-CH1 human chordoma)
Derivation
This cell line was established from a local recurrence of a sacrococcygeal after radiotherapy 4 years after initial surgery.
Clinical Data
56 years
male
Caucasian
Genes Expressed
overexpression of transcription factor T (brachyury)
Comments
U-CH1 is the first human chordoma cell line. It exhibits chordoma-like characteristics and has molecular, genetic, and morphological features typical of chordoma. This cell line was established from a local recurrence of a sacrococcygeal chordoma after radiotherapy 4 years after initial surgery. Chordoma is a rare slow-growing tumor type, and U-CH1 is a relatively slow-growing cell line. U-CH1 has a heterogeneous morphology consisting of physaliferous cells with mcinous intercellular substance, which represent typical chordoma features. The cells overexpress the transcription factor T (Brachyury) that is the most specific marker for chordoma. This cell line was accessioned with the support of the Chordoma Foundation, a non-profit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for the chordoma disease.
Complete Growth Medium

Iscove's Modified Dulbecco's Medium (IMDM; ATCC® No. 30-2005): RPMI-1640 Medium (ATCC® No. 30-2001) (4:1) + 10% FBS (ATCC® No. 30-2020) + additional 1% L-glutamine (ATCC® No. 30-2214)

Subculturing

Coating description: Dilute rat tail type I collagen (BD Biosciences, Catalog No. 354236) to 50 μg/ml. Add 7.5 ml coating buffer to flask and incubate at room temperature for one hour. Carefully aspirate remaining solution. Rinse flask 2 times to remove acid, using 1x DPBS. Coated flasks may be used immediately or stored at 2-8°C up to one week under sterile conditions.

Volumes used in this subculture protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 5.0ml Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 5.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 5.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in 10 ml fresh growth medium.
  7. Add appropriate aliquots of the cell suspension to new coated culture vessels.
  8. Incubate cultures at 37°C.
Cryopreservation
Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions
Temperature: 37oC
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

                TH01: 7
                D5S818: 11, 12           
                D13S317: 11, 13
                D7S820: 9, 12
                D16S539: 12, 13
                CSF1PO: 10, 11
                Amelogenin: X Y
                vWA: 17
                TPOX: 8, 11

Passage Number
30
Name of Depositor
Silke Bruderlein, Peter Moller
Deposited As
Homo sapiens
Year of Origin
August 17, 1998
References

Scheil S, et al. Genome-wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U-CH1. Genes Chromosomes Cancer 32(3): 203-211, 2001. PubMed: 11579460

Bruderlein S, et al. Molecular characterization of putative chordoma cell lines. Sarcoma 2010: 630129. Epub 2010 Dec 30. PubMed: 21253487

Presneau N, et al. Role of the transcription factor T (brechyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study. J. Pathol. 2239(3): 327-335, 2011. PubMed: 21171078

Liu X, et al. Establishment and Characterization of a Novel Chordoma Cell Line: CH22. J. Orthip Res. 30 (10): 1666-1673, 2010. PubMed: 22504929

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