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Human Coxsackievirus A6
Human Coxsackievirus A6
規格:
價格:
編號:B211548
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human Coxsackievirus A6
商品貨號
B211548
Classification
Picornaviridae, Enterovirus, Human enterovirus A
Agent
Human Coxsackievirus A6
培養基
Strain
Gdula
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1.0 mL per vial
Storage Conditions
-70°C
Images
ATCC VR-1801 Photomicrographs
Comments
Derived by adaptation of primary cell culture product ATCC® VR-165™ to RD cells (ATCC® CCL-136™).  ATCC® VR-165™ and ATCC® VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Effect on Host
CPE, cell rounding and death
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
HA Wenner
Source
Stool from 5-year-old child with sore throat, fever and leg muscle spasm, New York, 1949.
References

Dalldorf G. The coxsackie virus group. Ann. N.Y. Acad. Sci. 56: 583-586, 1953. PubMed: 13139263

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