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pOF216
pOF216
規格:
價格:
編號:B211556
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pOF216
商品貨號
B211556
Designations
pOF216
Depositors
F Hosoda, M Ohki
培養基
Biosafety Level
1
Vector Information
Size (kb): 12.5000000000000000
Vector: pOF216 (plasmid)
Promoters: Promoter lac
Construction: pKP1013, pUC13
Marker(s):spcR
Construct size (kb): 12.5
Features: insert detection: lacZ'
marker(s): spcR
promoter: lac
replicon: oriF
Applications
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.5; HindIII--12.5.
The EcoRI site is available for cloning but the lacPO N-terminal region will be deleted.
The polylinker is inserted in frame within the lacZ gene facilitating selection for blue and white colonies.
Ligating lambda cosR adapters onto the termini of potential inserts and cosL adapters onto the linearized vector enhances the step of circularization of large molecules.
This is a low copy number mini-F based plasmid vector with rare restriction sites in its polylinker for cloning inserts of up to 100 kb.
The depositor recommends adding spectinomycin to the media at a concentration of 20-30 ug/ml at the first establishment of spcR transformants and 15 ug/ml thereafter.
Media
ATCC Medium 1065 (see below) plus 15.0 mcg/ml tetracycline ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Hosoda F, et al. An F factor based cloning system for large DNA fragments. Nucleic Acids Res. 18: 3863-3869, 1990. PubMed: 2197597

Mori H, et al. Structure and function of the F plasmid genes essential for partitioning. J. Mol. Biol. 192: 1-15, 1986. PubMed: 3029390

Maki S, et al. An amber replication mutant of F plasmid mapped in the minimal replication region. Mol. Gen. Genet. 191: 231-237, 1983. PubMed: 6353162

Fumie Hosoda, personal communication

Shipped
freeze-dried
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