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pAqpZ plasmid in E. coli

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編號:B211572

品牌:Mingzhoubio

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RNA

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產品名稱

pAqpZ plasmid in E. coli

商品貨號

B211572

Designations

pAqpZ plasmid in E. coli

GenBank Number

U38664

培養基

Species

Escherichia coli (Migula) Castellani and Chalmers

Depositors

P Agre

Applications

ecause of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame.

Vector

Construct size (kb): 3.774

Insert

DNA: cDNA

Insert lengths(kb): 0.813

Gene product: aquaporin Z [aqpZ*]

Insert Size (kb)

0.813

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/NotI--2.9, 0.84; SalI--2.9, 0.84; XhoI--2.9, 0.84.

The insert contains the following restriciton sites (approximate kb from the 5'end): SnaBI--0.25; BsmI--0.39; PacI--0.69.

The insert in this clone contains the complete open reading frame for the E. coli aquaporin Z [aqpZ*] gene plus 100 bp of the 3' untranslated region.After the 3' UTR, a SalI and XhoI restriction site have been added.Because of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame.

Classification

Enterobacteriaceae, Escherichia

References

Calamita G, et al. Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli. J. Biol. Chem. 270: 29063-29066, 1995. PubMed: 7493926

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