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pNAK28

規(guī)格:

價格:

編號:B211754

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

pNAK28

商品貨號

B211754

Designations

pNAK28

GenBank Number

V01555

培養(yǎng)基

Species

Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus

Depositors

Johns Hopkins University, G Milman, Johns Hopkins University

Applications

produces protein nuclear antigen 1

Vector

Construct size (kb): 6.199999809265137

Insert

DNA: genomic

Insert lengths(kb): 2.236000061035156

Tissue: P3HR-1 cell line

Gene product: nuclear antigen 1 [EBNA-1]

Target Gene: nuclear antigen 1

Insert Size (kb)

2.236

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 2.4; BglI--4.9, 1.2; SmaI--3.7, 2.4.

pHE6 is a high-expression plasmid derived from pUC8. Inserts are expressed as run-on products initiated at lambda N. Synthesis is regulated by the cI857 repressor encoded by the plasmid.

Corresponds to nt 109241-111960 of the GenBank record and includes a portion of the EBNA coding region (109298-109872, the carboxy-terminal third.

Expresses a 28 kDa fusion protein composed of 36 amino acids from lambda N protein and linker and 191 amino acids of EBNA sequence. The protein is recognized by anti-EBNA antibodies and includes the DNA-binding domain that interacts with oriP.

This SmaI fragment is downstream of the triplet repeat region in the BamHI K fragment.

Classification

Herpesviridae, Lymphocryptovirus

References

Milman G. Expression of antigenic epstein-barr virus polypeptides in bacteria and their use in diagnostics. US Patent 5,256,768 dated Oct 26 1993

Milman G, et al. Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man. Proc. Natl. Acad. Sci. USA 82: 6300-6304, 1985. PubMed: 2412231

Baer R, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310: 207-211, 1984. PubMed: 6087149

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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