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pAcMP3/PKCeta
pAcMP3/PKCeta
規格:
價格:
編號:B211801
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAcMP3/PKCeta
商品貨號
B211801
Designations
pAcMP3/PKCeta
Species
Homo sapiens, human
培養基
Depositors
DJ Burns
Applications
in another host, produces protein protein kinase C, eta
Vector
Construct size (kb): 12.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAcMP3
Intact vector size:
Type of vector: plasmid
Vector end: XbaI
Vector end: XbaI
Cloning sites:
Polylinker sites: BamHI XbaI EcoRI NotI EagI PstI BglII
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
promoter: basic protein, ->
marker(s): ampR
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCH
Genomic copy number: unique
Gene name: protein kinase C, eta
Contains complete coding sequence?: Y
Tissue: keratinocytes
Type of DNA: cDNA
Insert end: NheI
Insert end: NheI
Insert size (kb): 2.0
Cross references:
Insert lengths(kb): 2.0
Tissue: keratinocytes
Gene product: protein kinase C, eta [PRKCH]
Target Gene: protein kinase C, eta
Insert Size (kb)
2.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 1.3, 0.55; BglII--11.0, 0.9; PvuI--6.0, 4.0, 1.5, 0.95; PvuII--3.2, 3.0, 2.4, 1.8, 1.4, 0.45; SacI--12.0.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.21, 1.75; BglII--1.43; PvuI--1.28; PvuII--0.12, 1.86; SacI--1.37.
Constructed using NheI adaptors. Most of the insert (all but approximately 250 bp) can be excised using BamHI, but the insert will be in two fragments- 1.2 and 0.55 kb.
Contains the complete coding sequence only.
References

Rice JW, et al. A comparison of large-scale Sf9 insect cell growth and protein production: stirred vessel vs. airlift. BioTechniques 15: 1052-1059, 1993. PubMed: 8292338

David J Burns, personal communication

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