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pbetaAct-CAT 9

規格:

價格:

編號:B211814

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pbetaAct-CAT 9

商品貨號

B211814

Designations

pbetaAct-CAT 9

Species

Gallus gallus, chicken

培養基

Depositors

N Fregien

Vector

Construct size (kb): 7.0

Insert

DNA: genomic

Insert lengths(kb): 4.300000190734863

Gene product: actin, beta [ACTB]

Insert Size (kb)

4.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--7.0; HindIII--4.3, 2.7; XhoI--7.0.

The 2.7 kb HindIII fragment contains the actin promoter and the partial actin genomic DNA. The 4.3 kb HindIII fragment contains the CAT gene, the SV40 splicing and polyadenylation sequences and the pUC18 vector.

Contains the beta-actin promoter with its CAAT and TATA sequences, 1.5 kb of upstream flanking sequence, the transcription initiation signal, exon 1 (untranslated), intron 1, and a small segment of exon 2.

SV40 promoter sequence deleted.

References

Fregien N, Davidson N. Activating elements in the promoter region of the chicken beta-actin gene. Gene 48: 1-11, 1986. PubMed: 3470237

B190086：pbetaAct-CAT 15

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