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pFSEC
pFSEC
規(guī)格:
貨期:
編號:B211825
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pFSEC
商品貨號 B211825
Designations pFSEC
Depositors EW Miles
Biosafety Level 1
Vector Information
Size (kb): 5.6799998283386230
Vector: pFSEC (plasmid)
Promoters: Promoter Ptrc
Construction: pSTR4-2
Marker(s):ampR
Construct size (kb): 5.679999828338623
Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3.
Overproduces yeast cystathionine beta-synthase (full length).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

Jhee KH, et al. Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J. Biol. Chem. 275: 11541-11544, 2000. PubMed: 10766767

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component of:ATCC 87809
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Shipping Information

?Freeze dried E. coli containing the plasmid.
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