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pVL941.GCRD21
pVL941.GCRD21
規格:
價格:
編號:B211826
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pVL941.GCRD21
商品貨號
B211826
Designations
pVL941.GCRD21
GenBank Number

M11080

培養基
Species
Homo sapiens, human
Depositors
Genzyme Corp., ML Bergh, Genzyme Corp.
Applications
in another host, produces protein glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase
Vector
Construct size (kb): 11.5
Insert
DNA: cDNA
Insert lengths(kb): 1.899999976158142
Gene product: polyhedrin [GBA]
Target Gene: glucosidase, beta; acid
Insert Size (kb)
1.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of Escherichia coli DH5 alpha containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--11.5; HindIII--4.0, 3.5, 1.9, 1.1, 1.0; PstI--11.0, 0.3; PvuII--3.5, 3.1, 2.4, 1.4, 0.7, 0.3.
The glucocerebrosidase insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.83; PstI--0.27, 0.56; SalI--1.25; BamHI--1.48.
Contains the complete coding sequence for glucocerebrosidase (nt 6-1556), plus 290 nt at the 3' end. Corresponds approximately to nt 490-2336 of the sequence record.
pVL941 is a transfer vector encoding polyhedrin and containing its complete 5' non-coding regulator sequence.
References

Rassmussen J, et al. Enzymatically active recombinant glucocerebrosidase. US Patent 5,236,838 dated Aug 17 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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