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pMF7 [BW72]
pMF7 [BW72]
規(guī)格:
貨期:
編號(hào):B211857
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pMF7 [BW72]
商品貨號(hào) B211857
Designations pMF7 [BW72]
Depositors M Feiss
Biosafety Level 1
Vector Information
Size (kb): 6.8000001907348630
Vector: pMF7 (cosmid)
Construction: pBR322, lambda
Marker(s):ampR
Construct size (kb): 6.800000190734863
Features: marker(s): ampR
replicon: pMB1
enhancer: none
Applications
vector for constructing genomic libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A general purpose cosmid vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Feiss M, et al. Cosmid DNA packaging in vivo. Gene 17: 123-130, 1982. PubMed: 6211394

Feiss M, Widner W. Bacteriophage lambda DNA packaging: scanning for the terminal cohesive end site during packaging. Proc. Natl. Acad. Sci. USA 79: 3498-3502, 1982. PubMed: 6285351

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
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