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pFH111
pFH111
規格:
價格:
編號:B211883
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pFH111
商品貨號
B211883
Designations
pFH111
GenBank Number

XO2761

培養基
Species
Homo sapiens, human
Applications
A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism.
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.899999976158142
Tissue: Hs578T cell line
Gene product: fibronectin 1 [FN1]
Alleles: A2, E2, A1, B2, D2, B1, D1, E1
Insert Size (kb)
1.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.8, 0.6; HindIII--5.6; BamHI--4.5, 0.7; HindIII/BamHI--3.0, 1.5, 0.7; EcoRI--3.6, 1.5.
A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism.
The 2.25/2.2 kb TaqI RFLP can be resolved on 1% agarose gels run for 20 cm.
Enzyme(s) not detecting polymorphism: HaeIII, HindIII, EcoRI, PstI, BglII.
References

Kornblihtt AR, et al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. PubMed: 2992939

Gardella R, et al. A new TaqI RFLP of the human fibronectin (FN1) gene. Nucleic Acids Res. 17: 10520, 1989. PubMed: 2574858

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