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pKMY266 [EcY5057, pKY266]plasmid in E. coli
pKMY266 [EcY5057, pKY266]plasmid in E. coli
規格:
價格:
編號:B211932
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKMY266 [EcY5057, pKY266]plasmid in E. coli
商品貨號
B211932
Designations
pKMY266 [EcY5057, pKY266]plasmid in E. coli
GenBank Number

M65106

M95045

培養基
Species
Pseudomonas mendocina Palleroni
Depositors
Amgen, Inc., K Yen, Amgen, Inc.
Applications
contains sequence toluene-4-monooxygenase
produces protein toluene-4-monooxygenase
Vector
Construct size (kb): 45.40
Insert
DNA: genomic
Insert lengths(kb): 20.4
Gene product: toluene-4-monooxygenase A [tmoA]
Insert Size (kb)
20.400
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 16.0, 2.4; HindIII--27.5, 4.4, 2.9, 2.6, 2.1, 1.7, 1.1, 0.82; SacI--24.0, 10.0, 9.8.
The complete coding sequence can also be isolated from partial digests of the clone as a 6.7 kb HindIII-BamHI fragment or a 4.7 kb HindIII-SmaI fragment.
Insert contains the following restriction sites approximate kb from the 5' end): BamHI--18.1; BglI--13.3, 14.5; EcoRI--16.9; HindIII--14.0, 14.8, 19.3; PstI--14.3, 14.6; SacI--10.2; SalI--12.2, 12.8; SmaI--16.1; XhoI--15.3, 20.1.
Insert contains two SacI fragments of 10.2 kb each. The downstream fragment encodes the six open reading frames involved in the expression of toluene-4-monooxygenase.
References

Yen KM, et al. Bioconversions catalyzed by the toluene monooxygenase of Pseudomonas mendocina KR-1. US Patent 5,171,684 dated Dec 15 1992

Yen KM, et al. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 173: 5315-5327, 1991. PubMed: 1885512

Yen KM, Karl MR. Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase. J. Bacteriol. 174: 7253-7261, 1992. PubMed: 1429451

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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