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pNOT19

規(guī)格:

價(jià)格:

編號(hào):B212010

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pNOT19

商品貨號(hào)

B212010

Designations

pNOT19

Depositors

HP Schweizer

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 2.6940000057220460

Vector: pNOT19 (plasmid)

Promoters: Promoter lac

Construction: pUC19

Marker(s):ampR

Construct size (kb): 2.694000005722046

Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1

Applications

vector permitting visual detection of recombinants

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PvuI--1.75, 0.9; HindIII--2.7; NotI--2.7.

Available for research purposes only.

General purpose cloning vector containing a unique NotI site. Constructed for insertion of mobilization cassettes pMOB1-4 (ATCC 77280-77283) to produce a vector useful for allele replacement in Pseudomonas aeruginosa.

Also permits visual detection of recombinants by lacZalpha complementation. The alpha peptide is 107 aa.

Media

ATCC^® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin

Growth Conditions

Temperature: 37.0°C

References

Schweizer HP. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol. Microbiol. 6: 1195-1204, 1992. PubMed: 1588818

Shipped

freeze-dried

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