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Size (kb): 6.2300000190734860

Vector: pRL124 (plasmid)

Promoters: Promoter none

Construction: pMC1403, pKO-100, M13 IG, trpA terminator, polylinker

Marker(s):ampR

Construct size (kb): 6.230000019073486

Features: insert detection: lacZ
marker(s): ampR
promoter: none
replicon: pMB1
terminator: trpA

promoter-cloning vector

vector containing primer sites useful for sequencing

vector permitting production of single-stranded DNA

vector permitting visual detection of activity in colonies

vector useful for in-vitro mutagenesis

Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.3; EcoRI--6.2.

This is a promoter-cloning vector with beta-galactosidase reporter gene, trp a terminator upstream of MCS, galK leader with stop codons in 3 reading frames, and intergenic region of M13 to permit ssDNA preparation for mutagenesis and/or sequencing.

RL724 (ATCC 37683) and RL726 (ATCC 37684) differ in having the M13 intergenic region in opposite orientations in a different sized fragment.

This plasmid was constructed from the galK leader of pKO100 fused to the lacZ of pMC1403; the M13 intergenic region of pZ152; the multiple cloning sites of M13mp19; and the trp a terminator of pRL118.

Temperature: 37.0°C