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pBH53
pBH53
規(guī)格:
價(jià)格:
編號:B212114
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBH53
商品貨號
B212114
Designations
pBH53
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RL White
Applications
Not recommended for detection of p53 transcripts.
The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies.
Vector
Construct size (kb): 6.800000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TP53
Genomic copy number: unique
Gene name: tumor protein p53 (Li-Fraumeni syndrome)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p13.1
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 4.1
Cross references:
Insert lengths(kb): 4.099999904632568
Gene product: tumor protein p53 (Li-Fraumeni syndrome)( tumor protein p53 (Li-Fraumeni syndrome), p53 cellular tumor antigen) [TP53]
Alleles: D2, C2, C1, A1, A1, B2, B1, D1, A2, A2
Insert Size (kb)
4.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.1, 2.7; BglI--3.9, 1.1, 0.9, 0.8; EcoRI--6.8; AvaI--3.9, 2.3, 0.5; KpnI--6.8.
Not recommended for detection of p53 transcripts.
The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies.
Subcloned from a cosmid isolated by hybridization with a cDNA for p53.
Enzymes(s) not detecting polymorphism: BglII, EcoRI, HindIII, MspI, PstI, PvuII, RsaI, TaqI.
References

Hoyheim B, et al. A BamHI-polymorphism is detected by a genomic p53-clone (pBHP53). Nucleic Acids Res. 17: 8898, 1989. PubMed: 2573883

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