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19-1 exon 4
19-1 exon 4
規格:
價格:
編號:B212204
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
19-1 exon 4
商品貨號
B212204
Designations
19-1 exon 4
GenBank Number

K02819

培養基
Species
Oryctolagus cuniculus, rabbit
Depositors
PN Marche, TJ Kindt
Applications
The exon 4 sequence is highly conserved, so this probe should detect all class I genes.
Vector
Construct size (kb): 3.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 0.36599999666214
Tissue: T-lymphocyte RL-5 cell line
Gene product: major histocompatibility complex, class I, gene R9 [RLA-A]
Insert Size (kb)
0.366
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2; HindIII--3.2; EcoRI/HindIII--2.7, 0.4; EcoRI/PstI--2.7, 0.4.
Corresponds to nt 2182 to 2548 of the reported sequence, beginning in exon 4 and extending into exon 5.
The exon 4 sequence is highly conserved, so this probe should detect all class I genes.
References

Marche PN, et al. Structure of a functional rabbit class I MHC gene: similarity to human class I genes. Immunogenetics 21: 71-82, 1985. PubMed: 3917974

Rebiere MC, et al. A rabbit class I major histocompatibility complex gene with a T cell-specific expression pattern. J. Immunol. 139: 2066-2074, 1987. PubMed: 3497986

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