| 產品名稱 |
phamSREBP-1 |
| 商品貨號 |
B212213 |
| Designations |
phamSREBP-1 |
| GenBank Number |
U09103
|
| Species |
Cricetulus griseus, hamster, Chinese |
| Depositors |
JL Goldstein |
| Vector |
Name of vector: pBluescript KS+
Intact vector size: 2.96
Type of vector: phagemid
Vector end: SalI
Vector end: NotI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
|
| Insert |
Genome: hamster, Chinese
Gene symbol: SREBP1*
Gene name: sterol regulatory element binding protein-1
Contains complete coding sequence?: U
Tissue: CHO cell line
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: NotI adapter
Insert size (kb): 3.83
Cross references: DNA Seq. Acc.: U09103; Nucleotides 1-3816 of the insert correspond to nucleotides 1-3816 of U09103.
|
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Related Products |
Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI/NotI--4.2, 3.0; EcoRI--7.3; HindIII--7.3; PstI--4.4, 1.15, 0.9, 0.4, 0.3; PvuI--5.8, 1.1, 0.4. |
| References |
Sato R, et al. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory binding protein-1 (SREBP-1). J. Biol. Chem. 269: 17267-17273, 1994. PubMed: 8006035
|
