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phamSREBP-1
phamSREBP-1
規格:
價格:
編號:B212213
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
phamSREBP-1
商品貨號
B212213
Designations
phamSREBP-1
GenBank Number

U09103

培養基
Species
Cricetulus griseus, hamster, Chinese
Depositors
JL Goldstein
Vector

Name of vector: pBluescript KS+
Intact vector size: 2.96
Type of vector: phagemid
Vector end: SalI
Vector end: NotI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Construction: pUC19
Host range: Escherichia coli


Features (with orientation and position when available):
enhancer:
none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none

Insert
Genome: hamster, Chinese
Gene symbol: SREBP1*
Gene name: sterol regulatory element binding protein-1
Contains complete coding sequence?: U
Tissue: CHO cell line
Type of DNA: cDNA
Insert end: Modification: SalI linker
Insert end: Modification: NotI adapter
Insert size (kb): 3.83
Cross references: DNA Seq. Acc.: U09103; Nucleotides 1-3816 of the insert correspond to nucleotides 1-3816 of U09103.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Distribution host: Escherichia coli HB101 (ATCC 33694)
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SalI/NotI--4.2, 3.0; EcoRI--7.3; HindIII--7.3; PstI--4.4, 1.15, 0.9, 0.4, 0.3; PvuI--5.8, 1.1, 0.4.
References

Sato R, et al. Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory binding protein-1 (SREBP-1). J. Biol. Chem. 269: 17267-17273, 1994. PubMed: 8006035

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