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pRML2
pRML2
規(guī)格:
價(jià)格:
編號(hào):B212232
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pRML2
商品貨號(hào)
B212232
Designations
pRML2
Depositors
F Spencer
培養(yǎng)基
Biosafety Level
1
Vector Information
Size (kb): 5.0999999046325680
Vector: pRML2 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescript + URA3 + ARSH4
Marker(s): ampR

Features:
marker(s): URA3
marker(s): ampR
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: ori
restriction site: SalI
coding sequence: TEL
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1; EcoRI--5.1; NotI--5.1; XbaI--5.1; EcoRI/XbaI--4.5, 0.5.
YAC cloning vector. The plasmid was from pBluescript, and includes a URA3 selectable marker, yeast origin of replication ARSH4, a distal polylinker to support plasmid rescue of insert ends, T7 promoter, and a cloning polylinker for library production.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993.

Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106

Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95

Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865

Shipped
freeze-dried
Shipping Information
in E. coli containing the plasmid
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