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Caviid herpesvirus 2
Caviid herpesvirus 2
規格:
價格:
編號:B212338
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Caviid herpesvirus 2
商品貨號
B212338
Classification
Herpesviridae, Unassigned
Deposited As
Deposited as Guinea pig salivary gland virus
培養基
Agent
Caviid herpesvirus 2
Strain
22122
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Submaxillary glands from a guinea pig
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Guinea pig lung (ATCC® CCL-158™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 9-21 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% or greater of the monolayer.
Name of Depositor
MJ Collins, Jr., Microbiological Associates, Inc.
Source
Submaxillary glands from a guinea pig
References

Hartley JW, et al. Serial propagation of the guinea pig salivary gland virus in tissue culture. Proc. Soc. Exp. Biol. Med. 96: 281-285, 1957. PubMed: 13485080

Cross References

Nucleotide (GenBank) : AB592928.1 Caviid herpesvirus 2 DNA, complete genome, strain: 22122

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