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pCEA1
pCEA1
規格:
價格:
編號:B212341
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCEA1
商品貨號
B212341
Designations
pCEA1
Species
Homo sapiens, human
培養基
Depositors
W Zimmermann
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.8299999833106995
Tissue: colon adenocarcinoma
Gene product: carcinoembryonic antigen [CEA]
Insert Size (kb)
0.830
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.54 + others; EcoRI--5.3; HindIII--5.3; BamHI--5.3; SalI--5.3.
The insert contains HinfI, HpaII, PstI, HincII, SstI, and PstI sites. The PstI sites at the ends were created by the cloning procedure.
This is a 5' end probe for CEA.
Open reading frame encoded in pCEA1 and pCEA2 contains 2 closely-related repeats of 178 amino acids each. pCEA1 contains repeat a and part of repeat b; pCEA2 contains repeat b and part of repeat a.
The 0.534 kb PstI fragment detects mRNA of 3.8 and 3.2 kb from mouse mammary tumors.
References

Zimmermann W, et al. Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure. Proc. Natl. Acad. Sci. USA 84: 2960-2964, 1987. PubMed: 3033671

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