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當(dāng)前位置: 首頁 > ATCC代理 > HFBCL09 B212366
HFBCL09
HFBCL09
規(guī)格:
價格:
編號:B212366
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HFBCL09
商品貨號
B212366
Designations
HFBCL09
GenBank Number

M85629

培養(yǎng)基
Species
Homo sapiens, human
Applications
The predicted product size is (bp): 127. The observed product size(s) is(are) (bp): 128 (human).
Vector
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D10F117S1E
Other gene symbols: D10S484E
Gene name: DNA Segment, numerous copies, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens calbindin, 28 kDa
Chromosome: 10; Localization: 10
Genome: Homo sapiens
Gene symbol: D14F117S2E
Gene name: DNA Segment, numerous copies, expressed
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: M85629; EST: EST02146; Primer: M13 Forward;
THC id: THC79399
DNA Seq. Acc.: T04987; EST: EST02875; Primer: M13 Reverse;
THC id: THC111607
Insert lengths(kb): 1.5
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: calbindin, 28 kDa [CALB]
Insert Size (kb)
1.5
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; PstI--3.1, 1.0, 0.5; HindIII--3.0, 1.5.
EST02146 can be detected in genomic DNA using the following oligonucleotides (5'-3'): TCCTTGGGTAGAACTGCACT and GCTAATCCGTCCAGACTCAT.
EST02146 matches GenBank accession RATCALBD28 with 87.79% identity over the sequence range: 146-227.
This sequence record is for rat calbindin, 28 kDa (CALB).
The predicted product size is (bp): 127. The observed product size(s) is(are) (bp): 128 (human).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

J C Venter, personal communication

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