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BK-4E

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編號:B212417

品牌:Mingzhoubio

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DNA

RNA

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產品名稱

BK-4E

商品貨號

B212417

Designations

BK-4E

Species

eukaryotic cells

培養(yǎng)基

Depositors

Board of Trustees of the University of Kentucky, A De Benedetti, Board of Trustees of the University of Kentucky

Applications

in suitable host, produces protein eukaryotic translation initiation factor 4E

Vector

Construct size (kb): 12.80000019073486

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: BK shuttle
Intact vector size: 11.414
Type of vector: phagemid
Vector end: XhoI
Vector end: BamHI
Cloning sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Polylinker sites: AatII XhoI EcoRV NotI BclI SpeI SrfI PacI SalI EcoRI BamHI
SacII
Construction: RDB-0
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: T/t-Ag, <-
replicon: BK papovavirus
marker(s): neoR, <-
promoter: SV40
enhancer: dioxin responsive cytochrome P-450
promoter for expression: MMTV, ->
promoter for in vitro transcription: SP6, ->
MCS: AatII/.../SacII, ->
promoter for in vitro transcription: T7, <-
replicon: f1, <-
marker(s): ampR, ->
replicon: pMB1
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: eukaryotic cells
Gene symbol: EIF4E
Other gene symbols: EIF-4E
Gene name: eukaryotic translation initiation factor 4E
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: BamHI linker
Insert size (kb): 1.4
Cross references:

Insert lengths(kb): 1.399999976158142

Gene product: eukaryotic translation initiation factor 4E [EIF4E]

Target Gene: eukaryotic translation initiation factor 4E

Insert Size (kb)

1.400

Media

LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--11.5, 1.4; BamHI--13.0; EcoRI--13.0; PstI--7.5, 3.8, 1.0; XhoI--13.0.

References

De Benedetti A, Rhoads RE. Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrrant growth and morphology. Proc. Natl. Acad. Sci. USA 87: 8212-8216, 1990. PubMed: 2122455

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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