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SMV-34

規格:

價格:

編號:B212425

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

SMV-34

商品貨號

B212425

Designations

SMV-34

GenBank Number

D00717

培養基

Species

Soybean mosaic virus

Depositors

WG Dougherty

Vector

Construct size (kb): 7.699999809265137

Insert

DNA: cDNA

Insert lengths(kb): 4.5

Gene product: nuclear inclusion proteinase [CI]

Insert Size (kb)

4.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.45, 2.15; BamHI--7.6; HindIII--4.55, 2.7, 0.35; EcoRI/HindIII--3.0, 2.25, 1.5, 0.6, 0.35; EcoRI/BamHI--3.2, 2.35, 2.15; PstI/SstI--4.5, 2.8.

GenBank accession D00717 corresponds to the 3' end of this insert, which extends approximately from nt 2800 to nt 7300 of the SMV genome.

Restriction sites flanking the insert at the 5' end are EcoRI, SacI, and KpnI and at the 3' end are BamHI, XbaI, SalI, PstI, SphI, and HindIII.

Classification

Potyvirus

References

Ghabrial SA, et al. Molecular genetic analyses of the soybean mosaic virus NIa protease. J. Gen. Virol. 71: 1921-1927, 1990. PubMed: 2212987

William G Dougherty, personal communication

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