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MKK1a
MKK1a
規格:
價格:
編號:B212556
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
MKK1a
商品貨號
B212556
Designations
MKK1a
GenBank Number

L05624

培養基
Species
Homo sapiens, human
Applications
in another host, produces protein mitogen-activated protein kinase kinase protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase
Vector
Construct size (kb): 6.454999923706055
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDNA I/Amp
Intact vector size: 5.000
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Polylinker sites: HindIII BamHI BstXI EcoRI EcoRV BstXI NotI XhoI SphI NsiI
XbaI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: CMV
marker(s): ampR
promoter: CMV, T7, SP6
replicon: polyoma, ColE1, M13
terminator: SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MKK*
Gene name: mitogen-activated protein kinase kinase
Contains complete coding sequence?: U
Tissue: T-lymphocyte
Type of DNA: cDNA
Insert 5' end: BamHI
Insert 3' end: EcoRI
Insert size (kb): 1.455
Cross references: DNA Seq. Acc.: L05624
Insert lengths(kb): 1.455000042915344
Tissue: T-lymphocyte
Gene product: mitogen-activated protein kinase kinase( protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), MAP kinase kinase) [MKK*]
Target Gene: mitogen-activated protein kinase kinase
Insert Size (kb)
1.455
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRV--5.0, 1.45; BglII--6.5; EcoRI--5.5, 1.0; PvuII--2.8, 1.95, 1.7; HaeII--3.6, 2.2, 0.7.
The insert contains the following restriction sites (bp from the 5' end): AflIII--719; AlwI--486; BanI--860; BglII--1120; BspMII--353; BsrI--1010; BstUI--15; EcoRI--512; HaeII--176; HgiAI--463; HpaI--91;
HphI--1314; PvuII--61; RsaI--405; SspI--1374; TaqI--680.
A probe from nt 1-516 of the insert detects a 2.6 kb mRNA in human thymus, brain, kidney, muscle, liver, intestine, heart, lung, spleen and EGF-stimulated A431 cells.
References

Seger R, et al. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases. J. Biol. Chem. 267: 25628-25631, 1992. PubMed: 1281467

Edwin G Krebs, personal communication

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