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pLS21 [1002]
pLS21 [1002]
規(guī)格:
價格:
編號:B212605
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pLS21 [1002]
商品貨號
B212605
Designations
pLS21 [1002]
Depositors
Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory
培養(yǎng)基
U.S. Patent
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
2
Vector Information
Size (kb): 8.1000003814697270
Vector: pLS21 (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pAR2192
Marker(s):ampR,tetR
Construct size (kb): 8.100000381469727
Features: marker(s): ampR, tetR
promoter: T7 (phi10)
replicon: pMB1, rep
Applications
expression vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
Comments
Strain is noncapsulated and never reverts to encapsulated form.
Should be treated as a class II biohazard.
The HindIII site is downstream of the T7 promoter.
Media
ATCC® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990

Lacks SA, et al. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J. Mol. Biol. 192: 753-765, 1986. PubMed: 2438417

Sanford A Lacks, personal communication

Shipped
freeze-dried
Shipping Information

Shipped: ?Freeze-dried Streptococcus pneumoniae containing the plasmid.

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