| 產品名稱 |
pLS21 [1002] |
| 商品貨號 |
B212605 |
| Designations |
pLS21 [1002] |
| Depositors |
Brookhaven National Laboratory, SA Lacks, Brookhaven National Laboratory |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
2 |
| Vector Information |
Size (kb): 8.1000003814697270 Vector: pLS21 (plasmid) Promoters: Promoter T7 (phi10) Construction: pAR2192 Marker(s):ampR,tetR Construct size (kb): 8.100000381469727 Features: marker(s): ampR, tetR
promoter: T7 (phi10)
replicon: pMB1, rep |
| Applications |
expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro |
| Comments |
Strain is noncapsulated and never reverts to encapsulated form. Should be treated as a class II biohazard. The HindIII site is downstream of the T7 promoter. |
| Media |
ATCC® Medium 1420: Trypticase soy agar with defibrinated sheep blood, sucrose and 0.5 mcg/ml tetracycline
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Lacks SA. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of Streptococcus pneumoniae. US Patent 4,960,707 dated Oct 2 1990
Lacks SA, et al. Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1. J. Mol. Biol. 192: 753-765, 1986. PubMed: 2438417
Sanford A Lacks, personal communication
|
| Shipped |
freeze-dried |
| Shipping Information |
Shipped: ?Freeze-dried Streptococcus pneumoniae containing the plasmid. |
