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pMT2ADA-VWF
pMT2ADA-VWF
規格:
價格:
編號:B212755
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMT2ADA-VWF
商品貨號
B212755
Designations
pMT2ADA-VWF
GenBank Number

X04385

培養基
Species
Homo sapiens, human
Depositors
Genetics Institute, Inc., RJ Kaufman, Genetics Institute, Inc.
Applications
in suitable host, produces protein von Willebrand factor coagulation factor VIII VWF (von Willebrand factor)
Vector
Construct size (kb): 13.83100032806397
Insert
DNA: cDNA
Insert lengths(kb): 8.574999809265137
Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) [VWF]
Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2
Target Gene: von Willebrand factor
Insert Size (kb)
8.575
Media
ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 3.4, 2.8, 1.5, 0.6; EcoRI--7.6, 3.9, 2.5; BglII--4.4, 4.4, 3.5, 2.3.
Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538.
May be transfected into Chinese hamster ovary (CHO) cells in the ADA selective medium.
The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, murine adenosine deaminase (ADA) cDNA sequence, a DHFR cDNA sequence and pBR322 sequences.
References

Kaufman RJ. Method for producing factor VIII:C and analogs. US Patent 5,198,349 dated Mar 30 1993

Kaufman RJ, et al. Selection and amplification of heterologous genes encoding adenosine deaminase in mammalian cells. Proc. Natl. Acad. Sci. USA 83: 3136-3140, 1986. PubMed: 3486414

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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