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HFBCC16
HFBCC16
規(guī)格:
價(jià)格:
編號(hào):B212778
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
HFBCC16
商品貨號(hào)
B212778
Designations
HFBCC16
GenBank Number

M78440

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 5.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDC27*
Genomic copy number: unique
Gene name: cell division cycle 27 (human homolog of S. pombe nuc2+ and
A. nidulans bimA)
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 q21-q24
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.6
Cross references: DNA Seq. Acc.: M78440; EST: EST00588; Primer: M13 Forward
Insert lengths(kb): 2.599999904632568
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: cell division cycle 27 (human homolog of S. pombe nuc2+ and A. nidulans bimA) [CDC27*]
Insert Size (kb)
2.600
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.6; PstI--3.0, 1.7, 0.8; HindIII--5.2, 0.3.
According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00588 may contain coding sequence: 101-151 (good).
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Tugendreich S, et al. Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag database. Proc. Natl. Acad. Sci. USA 90: 10031-10035, 1993. PubMed: 8234252

J C Venter, personal communication

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