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pMLB1034 plasmid in E. coli

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編號:B212863

品牌:Mingzhoubio

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RNA

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產品名稱

pMLB1034 plasmid in E. coli

商品貨號

B212863

Designations

pMLB1034 plasmid in E. coli

Depositors

ML Berman

培養基

Biosafety Level

Vector Information

Size (kb): 6.26

Vector: pMLB1034 (plasmid)

Promoters: Promoter none

Construction: pMC871, pBR322

Marker(s):ampR

Construct size (kb): 6.26

Features: insert detection: lacZ
marker(s): ampR
promoter: none
replicon: pMB1

Applications

promoter-cloning vector

vector permitting construction of fusion proteins

Comments

A plasmid vector for screening promoters by determining beta-galactosidase activity (lacZ).

Escherichia coli MC1000 = F- araD139 delta(ara, leu)7697 delta(lac)chi74 galU- galK- rpsL.

The cloning sites in this vector are 5' to a promoterless beta-galactosidase gene. The insert must provide the promoter, the ribosome binding site, and/or the ATG translation initiation codon for activity to be expressed.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Shultz J, et al. A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell 31: 227-235, 1982. PubMed: 6297749

Berman ML. Vectors for constructing hybrid genes. BioTechniques 1: 178-183, 1983.

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freeze-dried

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