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DT201 [pDT201]
DT201 [pDT201]
規(guī)格:
價(jià)格:
編號:B212923
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
DT201 [pDT201]
商品貨號
B212923
Designations
DT201 [pDT201]
GenBank Number

K01722

培養(yǎng)基
Species
Corynebacterium diphtheriae (Kruse) Lehmann and Neumann
Depositors
President and Fellows of Harvard College, JR Murphy, President and Fellows of Harvard College
Vector
Construct size (kb): 3.5
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Insert
Genome: Corynebacterium diphtheriae
Gene name: diphtheria toxin, fragment A
Contains complete coding sequence?: N
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 0.831
Cross references: DNA Seq. Acc.: K01722 Nucleotides 1-831 of the insert correspond to nucleotides 58-889 of K01722.
Insert lengths(kb): 0.831
Gene product: diphtheria toxin, fragment A
Insert Size (kb)
0.831
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Plasmid made from pUC8 and a small segment of the Corynebacteriophage beta tox-45 genome.Insert corresponds to nucleotides 57-888 of the genbank sequence K01722.
References

Leong D, et al. Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12. Science 220: 515-517, 1983. PubMed: 6403984

Murphy JR. Hybrid protein. US Patent 4,675,382 dated Jun 23 1987

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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