ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.2, 3.7, 2.7, 0.2; EcoRI--4.3, 3.2, 2.9, 0.3; SacI--8.0, 2.8.
The insert contains the following restriction sites (according to genomic position): XhoI--1642; BamHI--1926, 2148, 7265; EcoRI--2414, 2474, 5646, 6105; BglI--3408.
The clone is infectious.
Contains the compete genome, interrupted at the SacI site that occurs at nt 5822.
