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p13-1-82
p13-1-82
規(guī)格:
價(jià)格:
編號(hào):B212972
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p13-1-82
商品貨號(hào)
B212972
Designations
p13-1-82
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.649999976158142
Gene product: DNA Segment, single copy [D19S11]
Alleles: F1, F2, A1, A1, A2, A2, B1, B2, C1, C2, D1, D2, D3
Insert Size (kb)
1.650
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--4.6, 2.8; SalI--7.4; PstI--5.4, 1.49; HindIII--5.4, 1.95; EcoRI--5.4, 1.73.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The combined heterozygosity of the polymorphisms detected by p13-1-82 (ATCC 59004, 59005) and p13-2-21 (ATCC 59014, 59015) is 72%. Seven of 24 possible haplotypes occur with frequencies of 3-38%.
The MspI (A alleles) and the TaqI (C alleles) polymorphisms are at the same locus (alpha locus). This polymorphism is also detected by p13-1-25 (ATCC 59870).
p13-1-82 (ATCC 59004, 59005), p13-2-31 (ATCC 59014, 59015), and p13-1-25 (ATCC 59870, 59871) were subcloned from cosmid 1-13 (C1-13).
References

Bufton L, et al. Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. Am. J. Hum. Genet. 38: 447-460, 1986. PubMed: 3010711

Michael Litt, personal communication

M E Medof, personal communication

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