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pCu424CTR1-CYC1

規(guī)格:

價格:

編號:B213053

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

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產(chǎn)品名稱

pCu424CTR1-CYC1

商品貨號

B213053

Designations

pCu424CTR1-CYC1

Depositors

S Labbe, DJ Thiele

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 6.2280001640319820

Vector: pCu424CTR1-CYC1 (phagemid)

Promoters: Promoter for expression CTR1-CYC1

Construction: pRS424, CTR1-CYC1 promoter

Marker(s):TRP1,ampR

Construct size (kb): 6.228000164031982

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1

Applications

cloning vector

Comments

Restriction digests of the vector give the following sizes (kb): SacI--6.2; SacI/SpeI--5.9, 0.4.

CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.

References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452