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HHCPF54
HHCPF54
規格:
價格:
編號:B213103
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HHCPF54
商品貨號
B213103
Designations
HHCPF54
GenBank Number

M78093

培養基
Species
Homo sapiens, human
Applications
The predicted product size is (bp): 142. The observed product size(s) is(are) (bp): 284 (human).
Vector
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D15S180E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens microtubule-associated protein-5
Chromosome: 15; Localization: 15 q15(21.1)-q22
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: M78093; EST: EST01683; Primer: M13 Forward
Insert lengths(kb): 1.399999976158142
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: microtubule-associated protein-5 [Mtap5]
Insert Size (kb)
1.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; BamHI--4.2, 0.2; PstI--3.2, 0.75, 0.5; HindIII--4.4.
EST01683 can be detected in genomic DNA using the following oligonucleotides (5'-3'): GTCCAAAGGCCTAGTCAATG and ACGGAAGAAGTCAAGGTCAG.
EST01683 matches PIR accession A33645 with 62% identity and 80% similarity over the sequence range: 195-344.
This sequence record is for mouse microtubule associated protein 1b (Mtap-1b).
The predicted product size is (bp): 142. The observed product size(s) is(are) (bp): 284 (human).
These primers appear to amplify across an intron approximately 140 bp in size that occurs near nt 185 of GENBANK file HUMXT01683.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994

J C Venter, personal communication

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