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當(dāng)前位置: 首頁 > ATCC代理 > HHCPN85 B213128
HHCPN85
HHCPN85
規(guī)格:
價格:
編號:B213128
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HHCPN85
商品貨號
B213128
Designations
HHCPN85
GenBank Number

M78165

培養(yǎng)基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CALM3
Other gene symbols: PHKD
Gene name: calmodulin 3
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.3
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M78165; EST: EST01758; Primer: M13 Forward;
THC id: THC171455
DNA Seq. Acc.: M86171; EST: EST02703; Primer: M13 Reverse;
THC id: THC171455
Insert lengths(kb): 1.700000047683716
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: calmodulin 3 [PHKD]
Insert Size (kb)
1.700
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 1.5.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.7; BamHI--4.6; PstI--3.2, 1.0, 0.25.
The gene may actually be calmodulin 1, not calmodulin 3. EST sequences are too short to determine which it is.
EST02703 matches GenBank accession HUMCAMA with 96.06% identity over the sequence range: 161-313.
EST01758 matches GenBank accession HUMCAMA with 96.45% identity over the sequence range: 311-1.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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