The SnaBI site, in the intron of SUP4ochre, inactivates SUP4ochre.
A yeast artificial chromosome vector.
pYAC3 was constructed from pYAC2 by replacing the SmaI site in the SUP4 intron with the SnaBI site, and eliminating the SfiI and NotI sites flanking the SUP4 gene.
The HIS3 sequence is removed when producing the telomers by BamHI digestion.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825
