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pDP1648

規格:

價格:

編號:B213165

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pDP1648

商品貨號

B213165

Designations

pDP1648

GenBank Number

U65918

培養基

Species

Homo sapiens, human

Depositors

DC Page, R Reijo, LG Brown, R Saxena

Vector

Construct size (kb): 5.199999809265137

Insert

DNA: cDNA

Insert lengths(kb): 2.200000047683716

Tissue: testis

Gene product: DAZ homologue [DAZH]

Insert Size (kb)

2.200

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--3.2, 2.2; NdeI--5.3; SspI/XhoI--2.2, 1.0, 0.66 (doublet), 0.55, 0.28.

Insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.76; HindIII--0.39, 1.82; NdeI--1.68; NcoI--0.52; PvuII--0.28, 0.85; XhoI--0.18.

Digestion with XhoI and SspI released a 0.95 kb fragment containing the entire open reading frame.

References

Saxena R, et al. The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned. Nat. Genet. 14: 292-299, 1996. PubMed: 8896558

David C Page, personal communication

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