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當前位置: 首頁 > ATCC代理 > YEpGAL10-FLP [YEp51-FLP] B213212
YEpGAL10-FLP [YEp51-FLP]
YEpGAL10-FLP [YEp51-FLP]
規格:
價格:
編號:B213212
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
YEpGAL10-FLP [YEp51-FLP]
商品貨號
B213212
Designations
YEpGAL10-FLP [YEp51-FLP]
Species
Saccharomyces cerevisiae, 2 micron circle
培養基
Depositors
JR Broach
Applications
in another host, produces protein recombinase, site-specific
Vector
Construct size (kb): 7.699999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: YEp51
Intact vector size: 6.800
Type of vector: plasmid
Vector end: SalI
Vector end: HindIII
Cloning sites: HindIII SalI BamHI
Polylinker sites:
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, LEU2
replicon: pMB1, 2 micron
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae, 2 micron circle
Gene symbol: FLP
Genomic copy number: unique
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: HaeIII
Insert end: HindIII
Insert size (kb): 0.9
Cross references:
Insert lengths(kb): 0.8999999761581421
Gene product: recombinase, site-specific [FLP]
Target Gene: recombinase, site-specific
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.2 (doublet); HindIII/SalI--7.0, 0.9; SalI--7.8; PstI--6.8, 1.05; XbaI--7.8.
Plasmid to eliminate 2 micron circle plasmids and generate cir0 strains by overexpression of FLP.
References

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855

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