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pRR2119
pRR2119
規格:
價格:
編號:B213364
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRR2119
商品貨號
B213364
Designations
pRR2119
GenBank Number

K01999

培養基
Species
Rhodospirillum rubrum (Esmarch) Molisch
Depositors
CR Somerville
Applications
produces protein ribulose-1,5-bisphosphate carboxylase oxygenase
Vector
Construct size (kb): 6.0
Insert
DNA: genomic
Insert lengths(kb): 2.40
Gene product: ribulose-1,5-bisphosphate carboxylase oxygenase [RuBP]
Target Gene: ribulose-1,5-bisphosphate carboxylase oxygenase
Insert Size (kb)
2.400
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.5, 0.6; PstI--3.1, 2.0, 0.8; SalI--5.6, 0.3.
The insert contains the following restriction sites (kb from the EcoRI site 10 bp 5' to the BamHI/Sau3AI junction): SalI--0.26, 0.52, 0.85; PstI--1.0, 1.8.
Expression is regulated by the lac promoter, and is low in the absence of inducer.
References

Somerville CR, Somerville SC. Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli. Mol. Gen. Genet. 193: 214-219, 1984.

Nargang F, et al. Nucleotide sequence of the ribulosebisphosphate carboxylase gene from Rhodospirillum rubrum. Mol. Gen. Genet. 193: 220-224, 1984.

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