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pEagRM2.9 [pEag RM2.9]
pEagRM2.9 [pEag RM2.9]
規格:
價格:
編號:B213509
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pEagRM2.9 [pEag RM2.9]
商品貨號
B213509
Designations
pEagRM2.9 [pEag RM2.9]
Species
Pantoea agglomerans (Beijerinck) Gavini et al.
培養基
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
in suitable host, produces protein modification methylase EagI
in suitable host, produces protein restriction endonuclease EagI
Vector
Construct size (kb): 7.30
Insert
DNA: genomic
Insert lengths(kb): 2.90
Gene product: restriction endonuclease EagI [hsdM]
Target Gene: modification methylase EagI, restriction endonuclease EagI
Insert Size (kb)
2.900
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Original depositor-supplied purified DNA. Distributed in aliquots sufficient for transformation.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.5, 2.9; EcoRV--4.4, 1.5 (doublet): AvaI--4.1, 3.3; NotI--uncut.
The insert contains the following restriction sites (approximate kb from the end closer to the ori): EcoRV--0.8, 2.4; ClaI--1.2, 2.2; SacI--1.6; PvuII--2.7.
The vector is pBR322 modified such that the EcoRI site, the PvuII site, and the two DraI sites located outside of the bla gene have been replaced by NotI sites.
Distributed in aliquots sufficient for transformation.
References

Brooks JE, Sznyter LA. Method for producing the EagI restriction endonuclease and methylase. US Patent 4,996,151 dated Feb 26 1991

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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