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Acanthamoeba hatchetti Sawyer et al.
Acanthamoeba hatchetti Sawyer et al.
規(guī)格:
價格:
編號:B213540
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Acanthamoeba hatchetti Sawyer et al.
商品貨號
B213540
Strain Designations
4RE
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

培養(yǎng)基
Isolation
clinical specimen - human
Graz Austria
Isolation date: 1997
Product Format
frozen
Storage Conditions
liquid nitrogen vapor phase
Type Strain
no
Medium
ATCC® Medium 712: PYG w/ Additives
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
Interval: every month
Subcultivation
Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCC medium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days.
Interval: every month
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor
J Walochnik
Chain of Custody
J Walochnik
Year of Origin
1997
References

Walochnik J, et al. Discrimination between clinically relevant and nonrelevant Acanthamoeba strains isolated from contact lens- wearing keratitis patients in Austria. J. Clin. Microbiol. 38: 3932-3936, 2000. PubMed: 11060047

Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891

isolated from the contact lens case of a patient with non-acanthamoeba keratoconjunctivitis

Cross References

Nucleotide (GenBank) : AF019068 Acanthamoeba hatchetti BH-2 18S ribosomal RNA gene, partial sequence

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