Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI/PstI--4.0, 1.0; EcoRI/EcoRV--3.2, 1.8.
The resulting protein contains two additional amino acids at the amino terminus (Gly-Ser).
Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids.
The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.
Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine "kinker" and thrombin recognition site to enhance cleavage of the fusion protein.
