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pNF2
pNF2
規格:
價格:
編號:B213596
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pNF2
商品貨號
B213596
Designations
pNF2
Depositors
PA Scolnik
培養基
Biosafety Level
1
Vector Information
Size (kb): 7.8000001907348630
Vector: pNF2 (plasmid)
Promoters: Promoter nifH
Construction: pBR322, nifH, str/spcR
Marker(s):ampR,spcR,strR
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, spcR, strR
promoter: nifH
replicon: pMB1
terminator: none
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SalI--8.2; BamHI--8.2; EcoRI/PstI--4.2, 3.6.
The spcR marker is expressed in R. capsulatus.
A wide range of transcription levels can be obtained by varying the nitrogen source in the medium. Transcription can be immediately stopped by the introduction of oxygen and ammonia.
A Rhodobacter capsulatus expression vector with transcription of the insert under the control of the nifH promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Pollock D, et al. Transcription of the rhodobacter capsulatus nifHDK operon is modulated by the nitrogen source. Construction of plasmid expression vectors based on the nifHDK promoter. Gene 65: 269-275, 1988. PubMed: 3410321

Shipped
freeze-dried
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

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